ABSTRACT
OBJECTIVE@#To observe the changes of relative expression of myocardial various RNAs in rats died of different causes and their relationship with PMI.@*METHODS@#The rat models were established in which the rats were sacrificed by broken neck, asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium. The quantitative real time PCR was used to calculate threshold cycle values of RNAs including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, inducible nitric oxide synthase (iNOS), hypoxia-inducible factor-1 (HIF-1), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and U6 small nuclear RNA (U6 snRNA) and to study the changes of the relative expressions of various indexes with PMI.@*RESULTS@#U6 snRNA with stable expression level could be used as appropriate internal control. In the early PMI, the relative expression of GAPDH, HIF-1, iNOS, TNF-alpha, and IL-6 more characteristically increased in groups of asphyxia and hemorrhagic shock than in group of broken neck, but the quantity of beta-actin decreased in all groups. In the late PMI, all the relative expressions significantly declined in correlation with the degradation of RNA.@*CONCLUSION@#The characteristic changes of each RNA expression can be used as references to estimate PMI in deaths by different causes.
Subject(s)
Animals , Rats , Actins , Cause of Death , Cytokines/metabolism , Disease Models, Animal , Enzymes/metabolism , Glyceraldehyde-3-Phosphate Dehydrogenases , Myocardium/metabolism , Nitric Oxide Synthase Type II , RNA/metabolism , RNA, Small Nuclear , Shock, Hemorrhagic , Tumor Necrosis Factor-alphaABSTRACT
The purpose of this study was to investigate the effect of glutathione (GSH) on blood coagulation. The normal plasma samples and mixed plasma samples were taken randomly, and into which the normal dose and different concentration of GSH were added. The prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and thrombin time (TT) were detected by using coagulation method before and after treatment with GSH. The detection results of normal plasma and mixed plasma containing GSH of different concentration were compared and analyzed with linear regression. The results showed that the APTT and FIB values of the plasma containing 2.5 mg/L glutathione or more, PT values of the plasma containing 10 mg/L glutathione or more, and TT values of the plasma containing 1250 mg/L glutathione or more were significantly different from those results of normal plasma or mixed plasma (P < 0.01) . There was a linear relation between all of the detection results of PT,APTT, FIB, TT and glutathione concentrations. The results of TT, APTT, PT and FIB detection in patient plasma were statistically different (P < 0.01) before and after treatment with normal concentration GSH. It is concluded that glutathione can influence detection results of coagulation function.
Subject(s)
Female , Humans , Male , Blood Coagulation , Fibrinogen , Glutathione , Pharmacology , Partial Thromboplastin Time , Plasma , Prothrombin Time , Thrombin TimeABSTRACT
OBJECTIVE@#To determine the effect of radiotherapy on the thyroid of patients with nasopharyngeal cancer.@*METHODS@#Thyroid dynamic imaging was performed on 51 patients with nasopharyngeal cancer who had the metastasis of the jugular lymph node before and after the radiotherapy. The peak time of the thyroid artery perfusion and the constant K were obtained. The levels of free triiodothyronine (FT3), free thyroxine (FT4), and thyroid stimulating hormone (TSH) in the blood serum were measured at the same time.@*RESULTS@#The peak time of the left and right thyroid artery perfusion before the radiotherapy was (14.5+/-2.1)s and (15.1+/-1.9)s, respectively, while that after the radiotherapy was (19.3+/-3.2)s and (20.2+/-3.5)s, respectively. There was significant difference between the pre- and post-radiotherapy (P0.05, respectively].@*CONCLUSION@#The peak time of the thyroid artery perfusion and the constant K which reflect blood stream status after the radiotherapy are all damaged in patients with nasopharyngeal cancer. The level of FT3 and FT4 in the blood serum is dropped but the TSH level has no obvious change.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Hypothyroidism , Blood , Nasopharyngeal Neoplasms , Blood , Radiotherapy , Thyroid Function Tests , Thyroid Gland , Radiation Effects , Thyrotropin , Blood , Thyroxine , Blood , Triiodothyronine , BloodABSTRACT
Quorum sensing is defined as the cell density-dependent regulation of gene expression, and the involved system is the quorum sensing system, in which N-acyl homoserine lactone is known as the signal molecules of most gram-negative organisms. It can regulate diverse physiological functions. This paper reviewed the quorum sensing systems and the recent advances which play a major role in the formation of the symbiosis between the rhizobia and their host plants.